I was wondering till yesterday, why is it necessary to learn Thermodynamics in Biotechnology..!!! Got the answer today. Learning the fluid flow rates and heat transfer would help me better in industry when we design a fermenter; moreover, it would help in understanding the flow of bulk biological products through a pipe. Rotameter; Flow through a cylindrical straight pipe & annulus; Fluidisation, Exatraction... mmm... Some more things sir explained in the lab. Got to know the fourier's equation for heat transfer! ( Hope I'm right :) ) I love answering questions, whenever a lecturer questions, I would open my mouth, whether i'm right or wrong!!! I get corrected by doing so. Sometimes, this leads to funny situations when friends start laughing at me coz of my silly answers!! & sometimes due to my silly doubts!. But, I never mind, :P I dare to answer everything!!! I love to be an apple in the eyes of my lecturer :) :) :) :) I got your mind voice.. But, i don't care... I'l always blow my own trumpet!!!! :P :)
Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"??? :P) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
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