Students who are willing to join Biotechnology course., after their high school, will have this great doubt!!!! B.Sc or B.tech???!!! Which helps me better?!! As a B.Tech - Biotechnolgy student , here I shared what i know. B.tech is more industry oriented. But, B.sc. is more research oriented. It's not that if you go for B.tech you can't research! As, I'm studying I could say " In B.tech we deal about bio processing; thermodynamics; which are more industry based. But, I learn microbiology, biochemistry and similar papers too." What my professor suggested was " If anyone wanna research in biotechnology, it's better to go with B.sc., M.sc., Phd. ;But, B.tech., M.tech & Ph.d has high value in industry! ". It's hard to shine here in this field. Hard work & patience are much needed. But, once you get a master degree, your value gets multiplied!
Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"??? :P) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
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