Skip to main content

Posts

Showing posts from May, 2013

I'm in love!

As a biotechnology student whether I'm taking care of my meals plate or not, I have to take good care of my petriplates. I wash my petriplates better than my meals plate! Yes, we never autoclave and sterilise our meals plate, but we do for our petriplates! We ( I mean me and my friends) enjoy cleaning and autoclaving our petriplates in our  department media room. And you know, my friends always complaint that I'm never accompanying them in cleaning. Yes, I'll go, wrap my petriplates, put it in autoclave, but I find it very hard to wash! Always, I do autoclave, but, never wash after the autoclaving, I'll give that easy job to my friends :P I love my petriplates! Yes, I love them a lot. But, how do I say that I love my petriplates? I love them because I'm always with them. I love them because, even whether I do complex Genetic engineering or simple Microbiology, I always need them! And the final reason for loving my petriplates is they are so cute, especially,

Types of plasmid!

I learnt genetic engineering, and of course, about plasmids. But, when I was questioned about the types of plasmid, God, I was blinking! :( I had known plasmid only as a vector most times, I was aware of the types of vectors but, types of plasmids? :( Okay, It's not a great shame, I learnt it now! Let me share here about the types of plasmids, just what I learnt. Based on the functions of a plasmid they are classified as, Resistance Plasmids: These are the plasmids which have resistance genes. For example the gene coding beta lactamase which offers resistance against beta lactum antibodies like ampicillin. This is an important thing which is used while performing cloning experiments for checking the transformation. This was called as "R Factor" before the discovery of plasmids. Fertility plasmids (F Plasmids): And, these type of plasmids they have important genes called tra genes, never wonder, tra genes are transfer genes which are essential for the non sexua