The very first day in my laboratory, the first lab for smearing practice; I found it terribly hard to smear my slide with blood; Oh... I started begging my friends to smear my slide , after 18 continuous failure attempts; Finally, none helped me; Because , they too were struggling to smear!!! lol!!! Ya, only a few in the lab managed to smear correctly! myself like mohammed gazni tried attempting and got it "ok" at last in my 25th attempt! That too, we have to smear our slide & get it confirmed as right or wrong from our mam who was the in charge for the lab. Every time, when i shown her my slide, she just said " no., this is not good, repeat! you didn't get the tail region of the smear properly!". I feel like "grrrr....". I didn't lose hope; tried & tried , finally got it somewhat right! then i continued with lieshman staining! The staining came good. Then I went to my microscope in search of " eosinophils, basophils, neutrophils, monocytes ..." . "oooofff..." I didn't find an eosinophil in that sample till last; I was keenly searching...! Mam arrived and said at last " oh, you won't find Eosinophils and basophils that much easier!!! " . "oh gosh!, then, why I was searching for it at first , leaving all the others in the list!!!!" I go mad at times in my lab. But, anyways, biotech lab is fun filled with lively dancing cell's show!!! Searching something in my microscope , " I feel and enjoy like Hide & Seek" :D :D :D :D :P
Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"??? :P) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
nalla vela nan medicalum edukkala, biotechnologyum edukkala
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