My colorful valentine! !!!

Hii...!! This time I'm gonna share my colorful  valentine search! For the valentine's day, we use to wear some specific colors which has specific means. Let me explain you, how my valentine was. I wore a grey colored costume :) (because that was not in the list of valentine). And, I went in search  of  purple & pink & black!

purple (blue)  ----> not in love!
Pink ----> Gonna propose!
Black ---> hates love!

:) :) Why i was searching these colors?? Any guesses???  Kindly tolerate with me & proceed further reading!
After a good search, I found a lovely purple, pink and black! :) Let me explain you the situation!

We had first three hours of theory class on the valentine's day. After the theory hours, I was much excited and eager to go to our lab! Because , our lab hours are the most happy, enjoyable times!
okay, let me come to the point now, why i searched for those colors?? Not only myself, all my class mates  searched for these colors.. What's so special about these colors??

Kindly tolerate with me! I searched for pink, purple & black because... We stained our microbes using GRAM'S STAINING TECHNIQUE, NEGATIVE STAINING & SIMPLE STAINING for viewing under light microscope.

In Gram's staining bacteria stain either as purple or pink :) While in simple staining just blue; In negative staining it looks transparent in black back ground.

Staining procedure :

Simple staining : 

• Mainly used for viewing the shape and structure of cells under microscope!
• cells stain blue in color! 

Procedure: 

• Prepare your slide clean.
• Place your culture at a corner of the slide using loop, air dry and heat fix.
• Add Methylene blue to the slide and keep for a minute.
• wash of the dye after a minute, air dry and view under microscope. 
• Beautiful blue color  cells can be found.
• Morphology of the cells are noted generally using this type of staining.

Gram Staining : 

• Based on the differences in the cell wall of the bacteria, they can be classified as gram positive and negative. 
• Negative one's have a thin peptidoglycan layer while positive one has thick peptidoglycan layer.

Procedure : 

• slide is prepared with the culture as mentioned above and air dried and heat fixed.
• For a minute, crystal violet is added and washed after a minute with water.
• Then, Iodine ( mordant - helps in effective fixing of dye to the cells) is added and kept for 30seconds.
• Wash off gently with little water. 
• Then, 95% of ethyl alcohol is added and kept for 30 seconds (time limit is important)
• Then, counter stain using Saffranin. 
• Wash off with water gently, air dry and view under microscope.

Note : 

• Gram positive's ---> Stain purple
• Gram negative ---> Pink 
• Wash the stain only using distilled water,  that too very gently for a maximum of 10 to 15 seconds.
• Add ethyl alcohol only for 30 seconds because gram positive may become negative due to degradation of peptidoglycan layer. 

Negative staining : 

• Cells appear in dark black background as transparent bodies. (It looks so beautiful) 

Procedure : 

• Place 2 to 3 drops of Indian ink (nigrosin) over a clean slide.
• Add loop of culture to it and mix well. 
• Smear the mixture with another slide as you do blood smearing. (refer for blood smearing procedure  picture here) 
• Air dry the slide and view under microscope. 

:) With these colorful stains, my lovely valentine's lab came to a pleasant end. I completed my staining perfectly with out errors :) There was an excellent chemistry between my microbes and myself.  :P They proposed me love as I looked into them under microscope. :) :P :P 

Happy night! Tata.. gonna read about Green greeny algae for tomorrow's test. :) bye.......!