Skip to main content

Isolated!!! I loved it!!

   When your isolated from people, you will feel bad. But., I'm happy with isolation :D. Yeah., don't wonder!
Quadrant Continuous
I did STREAKING in my lab as I mentioned in my yesterday's lab (MY STREAKS). I went to my incubator and checked for the growth. I streaked  E.coli & Lacto bacillus. I thought my streaking was too bad to get isolated colonies. But., to my surprise I got isolated colonies. Showed my plates with enthu to mam.
She said, " mm good, you got isolated colonies".
I felt too happy.

What I know about streaking :

Purpose of streaking : 
Why to Streak?

1) To get isolated colonies. ( mostly bacteria )
2) To check the presence of microbes in a sample ( sometimes)

Types:


1) Quadrant streaking: The plate is divided into 4 quadrants and streaking is done.
2) Continuous streaking: Continuous streak made from top to bottom.


As I mentioned we are going to do streaking with 2g/100ml and 2.5g/100ml agar in  next lab. My microbiology lab , I love it the most. Gonna experiment !! Love you my teachers.


Quadrant discontinuous

Continuous streaks

Labels:

Comments

  1. LeoPawFebruary 1, 2012 at 7:37 AM

    Just came here through some random cliks and I'm excited after browsing through. I appreciate your passion to learn and your attitude. Keep going with the same zeal and one day the world will honor you for all that you have achieved.

    ReplyDelete
    Replies
    1. Kanmani RajanFebruary 1, 2012 at 7:41 AM

      Thanks a lot Mr.Leo. I love learning & experimenting. Just started now only with basics. A long way to go. Guide me and give suggestions. Happy to hear from you :)

      Delete
  2. Kanmani RajanFebruary 2, 2012 at 6:40 AM

    Thank you :)

    ReplyDelete

Post a Comment

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
Post a Comment
Read more

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...
11 comments
Read more

The Butterfly flew for the first time!

Hi dear sweet lovely reader, Didn't meet you for a long time... Just after complaining about the stipend problem in the last post, I actually wanted to write a lot here, but, couldn't find time (Such a lazy girl, I am!). As you might be knowing, the author (me) is the butterfly here :P And, I flew for the first time!! YEAH! I FLEW! I flew like a butterfly! It was an awesome experience flying for the very first time! (ow, no! I dint put a trans-gene in my body and fly after getting  a pair of wings, but with a passport and VISA). I'm this girl, full of dreams wishing to do "this", "that" and "all kind of stuffs that one can do". And, one among those dreams was to fly one day! TO FLY FREE!(I mean, not free of cost :P, but liberation) I always wanted to fly in those big, big airplanes, but, I always have avoided that mode of transport. Reason? Obviously, it would burn a big hole in my dad's pocket (actually he can afford if I wan...
10 comments
Read more