Skip to main content

How to start a Project?

Hi,
I was thinking deeply, that how to spend my semester holidays. Got an idea of doing a mini project in my department. Let me explain you how to start with a project or how i did start. Here we go...

Step 1:
Choose or find out your area of interest. I mean the subject your interested or your favourite subject.
You may wonder, how do i know, I hate all the subjects! But, don't worry. 
Choose the subject you feel more easy than others.

Step 2:
Read research papers online related to your area of interest. Just google and read random stuffs, you will get an idea. There are many research papers available online ( You can try online magazines for latest research trends)

Step 3:
While you were reading, something might have interested you. Read much about that topic. Try to think differently, and frame your own project.

Note:
 The above steps would suit perfect if you are a Biotechnology student.

If you find it difficult to start a brand new project, just try to continue the projects which were already done.


Plant Tissue culture in Test tube
I started reading many research papers, but, I found difficult to frame a new project. So, I consulted my professors and now continuing the project of one of seniors. The project is regarding "Pant Tissue Culture".

I'm getting addicted to laboratory nowadays. Working in the laboratory makes me happy! :) :) :D

Now, I'm in my college Computer center :) I came to centre after soaking all the bottles of Plant tissue culture lab in soap solution for decontamination. Have to wait here and go back there, to decontaminate :) :P After decontamination, I'l start working with my professor from tomorrow :)

I got some basic info regarding the project I'm gonna continue. I got project report of my seniors for reading. Reading is lovely, when I'm reading for doing a project :) :P

Reading and discussing with the professor are awesome. I'm happy! :D :) :)
Bye! Bye! Gonna decontaminate the bottles :P :) :D
Wish you loads of smile and happiness :) Cheers!

Comments

Popular posts from this blog

Genotyping, Phenotyping, Karyotyping!

We are going to discuss here about three different typing! They are genotyping, phenotyping and karyotyping. Before learning about them let us learn what is genotype, phenotype and karyotype!

Genotype refers to the genetic make up of an organism. Generally the genes of an organism. The genotype of an organism can be represented as BB or Bb or bb based on the gene. If a person is having two recessive CFTR genes, then he will be getting cytic fibrosis. Genotype of an organism has also effect over the phenotype. Thus, genotype is representing the alleles of a gene in general. Genotyping is generally done based on PCR or hybridization.

Phenotype refers to the visible characters like structure, color and also the biochemical characters. This is based on the phenotype. Phenotyping can be done using biochemical assays.

Karyotype refers to the number of structure of chromosomes in an organism. karyotyping is done by staining and visualising the cells under microscope.

Isolation of monocytes from PBMC (Peripheral Blood Mononuclear Cells) - Principle and protocol

Whenever I'm made to realise that I'm not clear enough or good at something, I try to make myself clear with it. It happened today, during my laboratory examination, I was asked to perform monocyte isolation from a given blood sample, but, unfortunately, I was not very clear with the principle behind it.(but, still I managed to complete the experiment as I know the protocol, but, knowing the principle behind each step of the protocol clearly is very important, isn't it?). But, nothing is wrong in it, I made myself clear with it now. That's good, right?

So, let me share with you some basic principle and protocol for isolating monocyte from blood sample.

For isolating monocytes, initially we must isolate PBMC (Peripheral bood mono nuclear cells) from the blood sample. Here, let us make few terminologies clear before starting with the principle.

Peripheral blood sample - It is the blood sample obtained from acral areas of body (in general, it is the blood collected from ha…

Extraction of Plasmid DNA - Principle behind usage of various reagents

Hi,
Have a good time :) Last time I had explained you the procedure for "electroporation" and now with those electroporated cells, you can extract the plasmid DNA.

I'm not going to share the exact protocol here, but the mechanism behind the protocol we use in our lab.

Generally, the following reagents are used for the extraction of plasmid DNA.,
1) STET buffer
2)Lysozyme
3)RNAase
4)Phenol chloroform
5) Alcohol
6) Sodium acetate

If you need the protocol, kindly mail me I'll send you the  protocol. Now, let us discuss the principle behind the usage of each of these reagents.

STET buffer - contains Sucrose which helps in maintaining osmotic pressure; Triton X which helps in cleaving the cell wall; EDTA which acts as a chelating agent; Tris HCl is used as buffer.

Lysozyme - cleaves the cell membrane and wall;

RNAase- As  the name indicates, it cleaves RNA

Phenol chloroform (buffer saturated- pH 8) - Phenol denatures proteins, Chloroform prevents oxidised phenol from bind…