Skip to main content

Cellular computing - Molecular computing - DNA computing

Hi,
DNA computing, this is a topic in which my professor asked me and my friend  to give a lecture. We gave our best and here you can find a summary of our lecture.

DNA computing: It's computing with the use of DNA, in stead of the normal microprocessors used.

Why DNA or cells or biological molecules for computation?
I could give you three major reasons:
1) Large storage capacity
2) High parallelism
3) Speed

Large Storage capacity: DNA could store large information comparing the normal storage devices used like CDs. If we store the information in DNA in CDs and arranga it like a thread. That thread could round the Earth by 374 times!!!! Can you imagine the capcity of DNA now?

High parallelism:  DNA could process in several ways parellely.

Speed:  All biological reactions happen with in seconds. So, using biological molecules can increase the speed of computing to a greater extent.

Okay, but, how biological molecules could be used for computing?
The basics behind the computing is the usage of logic gates.

AND , OR gates are used. Cells can also behave as logic gates.

This is possible by a technique called CID - Chemically Inducible Dimerization.

This tool takes advantage of natural biological mechanisms that
bring together two proteins into a complex in the presence of an
enzyme
• FRP and FKBP joined by rapamycin.GAI and GID1 by gibberellins.
• OR gate, FRB and GAI were bound together at the cell membrane,
while FKBP and GID1 were bound together floating freely in
the cell. Adding either rapamycin, gibberellins, or both to cells
brought the freely floating complex to the one at the cellular
membrane, linking up the matching proteins and triggering the
output signal.
• AND gate, the researchers placed just GAI at the cell membrane,
with just FRB and complexes of FKBP and GID1 free-floating in the
cell. This system required all four proteins to link up to produce
membrane ruffling, which wouldn't occur without both input
chemicals.

This look great isn't it?

DISADVANTAGES of this DNA computing:
• Just newly developing
• Some problem regarding incorporating the
circuit into the host
• Need a manual control

I had a great time during the lecture session with my friend Priya :)
We confused our class mates as much as possible :P :P

Any doubts? kindly comment, I'll try to answer. Bye.. Take care!

Comments

Popular posts from this blog

Genotyping, Phenotyping, Karyotyping!

We are going to discuss here about three different typing! They are genotyping, phenotyping and karyotyping. Before learning about them let us learn what is genotype, phenotype and karyotype!

Genotype refers to the genetic make up of an organism. Generally the genes of an organism. The genotype of an organism can be represented as BB or Bb or bb based on the gene. If a person is having two recessive CFTR genes, then he will be getting cytic fibrosis. Genotype of an organism has also effect over the phenotype. Thus, genotype is representing the alleles of a gene in general. Genotyping is generally done based on PCR or hybridization.

Phenotype refers to the visible characters like structure, color and also the biochemical characters. This is based on the phenotype. Phenotyping can be done using biochemical assays.

Karyotype refers to the number of structure of chromosomes in an organism. karyotyping is done by staining and visualising the cells under microscope.

Isolation of monocytes from PBMC (Peripheral Blood Mononuclear Cells) - Principle and protocol

Whenever I'm made to realise that I'm not clear enough or good at something, I try to make myself clear with it. It happened today, during my laboratory examination, I was asked to perform monocyte isolation from a given blood sample, but, unfortunately, I was not very clear with the principle behind it.(but, still I managed to complete the experiment as I know the protocol, but, knowing the principle behind each step of the protocol clearly is very important, isn't it?). But, nothing is wrong in it, I made myself clear with it now. That's good, right?

So, let me share with you some basic principle and protocol for isolating monocyte from blood sample.

For isolating monocytes, initially we must isolate PBMC (Peripheral bood mono nuclear cells) from the blood sample. Here, let us make few terminologies clear before starting with the principle.

Peripheral blood sample - It is the blood sample obtained from acral areas of body (in general, it is the blood collected from ha…

One year into PhD - my BTS experience!

Dear readers,
I apologize that I stopped writing since last year. I don't have any great excuse other than poor planning. I was lost on my way to understand marine biology. As you might be knowing, I switched field, moved from my focus over bacteria and human disease related studies to climate change and marine biology. However, my research focus continues to be in molecular biology. Long story short, I was learning all about climate change, ocean acidification, ocean, marine animals in the past year. I even learnt how to spawn and culture oysters. How cool is it? Not cool?! Well, I think it is cool! 
I can setup mini Ocean acidification systems which mimics the future acidified ocean conditions; I even gained some plumbing related skills. This was totally a new experience and I thoroughly enjoyed it. However, I had a tough summer in a rural fishing village in China, learning how to grow oysters from the Chinese oyster farmers (for those who do not know what I am doing: I am doin…