Skip to main content

Genotyping, Phenotyping, Karyotyping!

We are going to discuss here about three different typing! They are genotyping, phenotyping and karyotyping. Before learning about them let us learn what is genotype, phenotype and karyotype!
Genotype, phenotype and karyotype

Genotype refers to the genetic make up of an organism. Generally the genes of an organism. The genotype of an organism can be represented as BB or Bb or bb based on the gene. If a person is having two recessive CFTR genes, then he will be getting cytic fibrosis. Genotype of an organism has also effect over the phenotype. Thus, genotype is representing the alleles of a gene in general. Genotyping is generally done based on PCR or hybridization.

Phenotype refers to the visible characters like structure, color and also the biochemical characters. This is based on the phenotype. Phenotyping can be done using biochemical assays.

Karyotype refers to the number of structure of chromosomes in an organism. karyotyping is done by staining and visualising the cells under microscope.

Comments

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...

Fire in the lab!!! BE CAREFUL!!!! A Lesson learnt!

   That day, we were working in our lab under laminar air flow chamber. we were streaking our plates with E.coli. For sterilizing the loop, we were using 70% ethanol. We also sterilized the L rod which we used for spreading , using the same Ethanol.  One by one, we started streaking and spreading. I completed my turn, and sterilized the L rod by dipping in ethanol once and shown in flame, and placed it in a tray.Mam instructed, " Ethanol will catch fire , if you place the hot rod over it" . One of my friend, did spreading after my turn, she too dipped the rod in ethanol, flamed the rod and instead of placing in the tray she misplaced the hot rod in the ethanol plate!!!! " oooo... fire fire..." immediately ethanol caught fire..!!! My friend who misplaced., she started crying... Mam shouted, " hey girls, guys run out of the lab...," as the tube which carries LPG for the flame was very close to fire. Suddenly, our lab technician acte...