Wednesday, August 1, 2012

Plant Tissue Culture medium


Today am gonna give certain tips for Plant tissue(PT) culture medium preparation.
The main parameter to be considered in plant tissue culture medium preparation is "Sterility". Your medium has to be very sterile. I could say, when you complete your medium preparation, 75% of your project or experiment is over.

I received a mail questioning the "Alternative for inositol"

Inositol - This is added as a stimulator to the PT medium. This is not a "must" component. But, this in certain plants promote growth.

Image courtesy - Google
The essential component for promoting cell growth is Thiamin. It's a vitamin which is basically required for growth of most plants. It is normally used at concentrations ranging from 0.1 to 10.0 mg/liter.

Coconut water, orange juice, malt extracts etc., are also added to PT medium as organic supplements.

You know better alternative for inositol? Then, Comment, share your knowledge :)

Wish you a happy day :)

(This suggestion is based on my theoretical and practical knowledge. This may  have discrepancy)

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