E.coli VS Plants! As an undergraduate of Biotechnology, I know about cloning . I know about cloning a gene which codes for a human protein into a bacterial cell, E.coli, most times. I also know about producing transgenic plant varieties. But, I don't know about cloning a human gene into plant! I came to know about cloning a human gene into plant and producing the protein in plants from the workshop session of AVIDADHAM'13. Let me explain something about this! As you may know, eukaryotic gene has both introns and exons. So cloning a eukaryotic gene into a prokaryote will have lots of problem like splicing and post translational modification. Generally for cloning eukaryotic gene into a prokaryote like E.coli, we do follow bottom up approach where we use mRNA to synthesise cDNA, to remove the noncoding introns. But, even if we remove the introns there will be problems with post translational modification. So, to avoid this, we are cloning our gene from human into pla...
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