Skip to main content

A search for my little friends in soil!!! :P

Mm... I know to isolate the microbial friends from soil and milk samples :)))  Learnt it in my lab :) tried it and found the results good. We used just a random soil sample. First the soil was diluted in 100 (10^-2) ml of water. Then serial dilution was made till we reached 10^-6. then using spread plate and pour plate methods we inoculated our microbial soil samples.  If milk, 1ml of sample diluted in 100ml at first. then serial dilution till 10^-6. The plates were incubated at 35 degree celsius for 24 hours.

Observation: 
Next day we found colonial growths in our plates :) Majority of the colonies were circular & punctiform (small circles). Some erose type(irregular) were also observed.

As the dilution increases, the colony number decreases. I learnt, "Pure culture can be isolated by dilution - Serial dilution".



 This is a basic thing in microbio lab. But, the happiness i experienced with this can't be explained. I felt as if i were a scientist :P. I enjoy every lab hour and  I'm much interested in these  lab works.   Wooo... a lovely lovely department. Can live my life with this subject. :)))))) Thank you dad for suggesting me this course.

     "Discovering the minute microbial buddies!!!! in my microscope!!"




Labels

Labels:

Comments

  1. UnknownFebruary 3, 2012 at 8:43 AM

    nice share ....

    ReplyDelete
  2. Ratna TanjungApril 5, 2012 at 6:42 AM

    i really love microbiology lab, but i don't really love chemical lab because the chemical pattern make me dizzy -_-'

    ReplyDelete
  3. Kanmani RajanApril 5, 2012 at 8:29 AM

    Happy learning with microbes :) @Ratna Tanjung

    ReplyDelete

Post a Comment

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
Post a Comment
Read more

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...
11 comments
Read more

Fire in the lab!!! BE CAREFUL!!!! A Lesson learnt!

   That day, we were working in our lab under laminar air flow chamber. we were streaking our plates with E.coli. For sterilizing the loop, we were using 70% ethanol. We also sterilized the L rod which we used for spreading , using the same Ethanol.  One by one, we started streaking and spreading. I completed my turn, and sterilized the L rod by dipping in ethanol once and shown in flame, and placed it in a tray.Mam instructed, " Ethanol will catch fire , if you place the hot rod over it" . One of my friend, did spreading after my turn, she too dipped the rod in ethanol, flamed the rod and instead of placing in the tray she misplaced the hot rod in the ethanol plate!!!! " oooo... fire fire..." immediately ethanol caught fire..!!! My friend who misplaced., she started crying... Mam shouted, " hey girls, guys run out of the lab...," as the tube which carries LPG for the flame was very close to fire. Suddenly, our lab technician acte...
3 comments
Read more