Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"??? :P) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...
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HOD once desperately commented on our pipetting skills.. like "ungala pipette panna sonen, cycle-ku kaathu adika sollala" nu, the whole class went into laughter...!
ReplyDeleteHaha.. :D :D We too do such funny things while pipetting. in biochemistry lab we use ml pipettes. Mam always comments " You need 4 persons for pipetting a solution., one for holding the beaker, one noting the level in the pipette; another holding the test tube ; one more saying "ae podhum podhum 0.5 dhan.." :P :)))
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