Skip to main content

DNA - RNA- Protein!

Cracking the genetic code! You know? Your DNA is made up of A,T,G,C (adenine, guanine, cytosine, thymine) These four letter are repeated through out the sequence. This DNA codes for Protein to be specific amino acids.

How your DNA is decoded?
Your DNA undergoes translation, that is, it gets converted as RNA and further this RNA is converted into proteins by transcription.

Translation? How it happens? DNA is a double helix as we all know. This double helix unwinds and now it has two trands, right? one strand is called a codon strand and the other is anti codon.

The codon strand is the coding strand. So, you think that you mut translate this codon strand? But, it is not certainly. The RNA is formed "complementary" to the anticodon strand!

You think, why? Here we go with the explanation. The word "complementary" has the importance here. Both the DNA strands are complementary to each other. As we know, the DNA has four nucleotide repeated through out namely ATGC.

Example of a complementary DNA strand:

ATGCTTGCGACT ---> Coding strand
TACGAACGCTGA ---> Anticodon strand

The coding strand has the exact details. But, the RNA is going to be formed in the complementary basis, so, the anti codon strand must be translated as follows.

ATGCTTGCGACT ---> Coding strand
AUGCUUGCGACU ---> RNA formed from anticodon by RNA polymerase enzyme.
TACGAACGCTGA ---> Anticodon strand

Note the imilarity between the coding trand and the RNA formed. RNA don't have "T", but, "U" instead. Here, by translating the anti codon strand we get the exact copy like the coding strand.

Further this RNA is trancribed as proteins. Each 3 letter set of the RNA codes for an amino acid of the protein.


This table describes the three letter coding format.

In this way your genetic code is cracked and information is extracted out. If there is any error in the code, then there will be error in the amino acid synthesis leading to Genetic diseases!

:) I hope you can understand this with little basics of biology. I tried my best to explain it simple. Doubts? Then comment! :)

Have a good time!

Comments

Post a Comment

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...

Fire in the lab!!! BE CAREFUL!!!! A Lesson learnt!

   That day, we were working in our lab under laminar air flow chamber. we were streaking our plates with E.coli. For sterilizing the loop, we were using 70% ethanol. We also sterilized the L rod which we used for spreading , using the same Ethanol.  One by one, we started streaking and spreading. I completed my turn, and sterilized the L rod by dipping in ethanol once and shown in flame, and placed it in a tray.Mam instructed, " Ethanol will catch fire , if you place the hot rod over it" . One of my friend, did spreading after my turn, she too dipped the rod in ethanol, flamed the rod and instead of placing in the tray she misplaced the hot rod in the ethanol plate!!!! " oooo... fire fire..." immediately ethanol caught fire..!!! My friend who misplaced., she started crying... Mam shouted, " hey girls, guys run out of the lab...," as the tube which carries LPG for the flame was very close to fire. Suddenly, our lab technician acte...