Skip to main content

My Exam#1

Hi,
Wish you good time. My Semester exams started. Today I wrote my favorite paper "microbiology". The question was easy. But, the answers were only little tough :P Anyways, I managed to do well. :)

There in the question paper, I had a wonderful question! I started thinking, "cha, we had never thought like this..!" The question was very simple, but, I didn't know the answer. Even now I'm not sure about the answer.
File:Dark Field Microscope.png
I had been using microscopes, mostly optical - bright field microscopes over a past few years, may be from my 11 th class I can say. I had read about Dark field microscope too. But, I had never thought of converting a bright field microscope to a dark field one! But, this question paper setters, really they are great. I felt  like saying, "what an idea sir ji", on seeing the question. :P :P :D

The question was, "can you convert a bright field microscope to a dark field microscope? If so how?"

I don't know the answer for this question. I thought like " yes it may be possible, but.. how? something like changing the light source position?"

I left space and proceeded with the next question. After finishing all the questions, I came to the space. 

I wrote like this:

" In bright field microscope, we get dark images over bright background; In dark field, bright images in dark background. Yes it is possible to convert a bright field microscope to dark field by changing the position of the light source" :P :P :P

I know this is wrong, but, anyways I filled the gap! Came home and searched for the answer. But, I didn't get the correct answer. 

The correct answer might be:

" Conversion is possible by changing the condenser lens of the bright field microscope, because dark field uses special lenses" 

I'm not sure with this. You know the answer? Can you give me the right answer? 

If your are a science person, who knows about microscopes, kindly answer me! :( :( 

Comments

  1. I think question paper is in "British English..." :p

    good that you love your subject :)

    ReplyDelete

Post a Comment

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...

Agarose gel Electrophoresis with principle

Hi, Agarose gel electrophoresis In the previous post I explained how to quantify DNA, and now we can find the molecular weight of that extracted DNA. But, how? Simple, you need to run an agarose gel! Preparation of agarose gel: Agarose gel must be prepared based on the DNA we are going to run. For example: if you are going to run Genomic DNA, you must use 0.6%gel, for plasmid DNA, you must use 0.8% gel, for PCR(Polymerase chain reaction) sample, you must use 1.2% gel. You may ask, why three different % of gels? The answer for your question is: Genomic DNA will be bulkier than pDNA - so, we must use a gel with less percentage, so that the pore size will be greater and the genomic DNA can move easily. In case of PCR sample, the gene amplified won't be bulky, so, it moves fast, very fast in low percent gel, that's why to have an optimum speed we use a higher percentage of gel comparatively. And, when our aim is to separate two sequences with relatively equal size...