Skip to main content

Isolated!!! I loved it!!

   When your isolated from people, you will feel bad. But., I'm happy with isolation :D. Yeah., don't wonder!
Quadrant Continuous
I did STREAKING in my lab as I mentioned in my yesterday's lab (MY STREAKS). I went to my incubator and checked for the growth. I streaked  E.coli & Lacto bacillus. I thought my streaking was too bad to get isolated colonies. But., to my surprise I got isolated colonies. Showed my plates with enthu to mam.
She said, " mm good, you got isolated colonies".
I felt too happy.

What I know about streaking :

Purpose of streaking : 
Why to Streak?

1) To get isolated colonies. ( mostly bacteria )
2) To check the presence of microbes in a sample ( sometimes)

Types:


1) Quadrant streaking: The plate is divided into 4 quadrants and streaking is done.
2) Continuous streaking: Continuous streak made from top to bottom.


As I mentioned we are going to do streaking with 2g/100ml and 2.5g/100ml agar in  next lab. My microbiology lab , I love it the most. Gonna experiment !! Love you my teachers.


Quadrant discontinuous

Continuous streaks

Comments

  1. Just came here through some random cliks and I'm excited after browsing through. I appreciate your passion to learn and your attitude. Keep going with the same zeal and one day the world will honor you for all that you have achieved.

    ReplyDelete
    Replies
    1. Thanks a lot Mr.Leo. I love learning & experimenting. Just started now only with basics. A long way to go. Guide me and give suggestions. Happy to hear from you :)

      Delete

Post a Comment

Popular posts from this blog

Lowry Assay Principle and procedure

Though there are several protein assays available, the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.01 mg/ml to 1 mg/ml. And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) Why Lowry?   Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, cost effective, easy to perform. Biuret assay is generally used for higher protein concentrations like tissue samples but, Lowry for less concentrated samples and hence used in most of the molecular biology laboratories where there will be need for assaying comparatively less concentrated protein samples (in most cases where we attempt to produce enzymes). Oth...

Sea Butterfly!

Neih hou!  Don't roll you eyes wondering what it is! "Neih hou" is how you say "hello" in Cantonese. Guess what, I am in Hong Kong and therefore the language Cantonese. I came to Hong Kong this summer as an intern before officially joining as a PhD Student. This is my second experience abroad, far away from home, new language, new culture, I expected me to have a "really" bad cultural shock, but, actually I experienced only 50% of what I expected. For a girl who have used the marina beach only for eating "sundal", bikini in beach was a shock (FEEL FREE TO JUDGE ME!). The first time, I went to the big wave beach here, I was the only one who was totally covered, when everyone was enjoying their Beer, I was slowly sipping my lemon juice (THE ODD ONE OUT!). I realized how beautifully different the world outside is! There is no single standard for right or wrong, it varies in different countries, in different regions around the world. And it ...

Agarose gel Electrophoresis with principle

Hi, Agarose gel electrophoresis In the previous post I explained how to quantify DNA, and now we can find the molecular weight of that extracted DNA. But, how? Simple, you need to run an agarose gel! Preparation of agarose gel: Agarose gel must be prepared based on the DNA we are going to run. For example: if you are going to run Genomic DNA, you must use 0.6%gel, for plasmid DNA, you must use 0.8% gel, for PCR(Polymerase chain reaction) sample, you must use 1.2% gel. You may ask, why three different % of gels? The answer for your question is: Genomic DNA will be bulkier than pDNA - so, we must use a gel with less percentage, so that the pore size will be greater and the genomic DNA can move easily. In case of PCR sample, the gene amplified won't be bulky, so, it moves fast, very fast in low percent gel, that's why to have an optimum speed we use a higher percentage of gel comparatively. And, when our aim is to separate two sequences with relatively equal size...